Well we know your male carries this gene and so does her bitch. Dont know who she bred her bitch to but the pups could be gene carriers. I am so glad you are helping her. I too have made many mistakes. One being I bred Manolo who has MVD to my bitch and gave the pups away to my good friends. So I am speaking with experience. All of the pups and mom and dad have been neutered and spayed. But I still worry that one will become sick and it would be my fault. Just saying...This cycle needs to stop...