i left maggies on for a couple of days each time, even at night. she didn't seem to bother with it tho. i should have left hers on longer, as one of her ears does not stand up right. it did for a very long time, but then during teething went back down. i taped again and it just never stood up right. so i would say 3 days minimum and leaving it on at night should be fine as long as they are not messing with it too bad.
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