Hi and welcome to YT
. If you lost just a little of it, I personally would not worry about it and would consider the vaccine potent. Over vaccination is one of the worst things you can potentially do to your dog's long term health, which is why I wouldn't give yet another vaccine.
Can I ask why you're administrating your own vaccines? My bach degree is in nursing and I don't even give my own vaccines, even though I know how to start an IV and such. I'm worried you're not quite giving it correctly bc I've never seen one of my dogs yelp when it's given by a vet. I would highly advise you let a professional give it, imo.