I begin neurological stimulation at 2 weeks old and have on every litter (except for the cold I do not do). Beyond the neuro standardly my pups have daily belly rubs, paw rubs and ear rubs and eye rubs. Then I add simple grooming at three weeks "ears, nails and bum shaves and light brushing." By six weeks they are introduced to water, simple baths, hair dryer, and ear cleaning; by the time the pups leave they're so accustom to the human touch and all the fussing that comes along with it