I used 3 bottles - two of them were the $50 size and saw no improvement in Cesar's eyes at all. I use the nursery water and I have a water filter for the house - still nasty eyes. I bought some grain free food today; hopefully that will help. I use Spa facial scrub, eye cleaning pads, saline, and still have stains. He is a golden yorkie so the stains are more noticeable.