Yes, females will mark their territory, spayed or not...what I would do..is talk to her ask her if she has to potty, than say to her, lets go outside and potty and keep saying that to her until you are outside, than say go potty hurry up...I would also take her outside every few hours and on the way out, talk to her say lets go outside and potty...Yorkies are very smart, she will pick up on it and soon will start coming to you and either bark at you or just sit and look at you...mine bark at me, and will not stop until I take them outside to potty...and when she does potty outside throw a potty party, tell her what a good girl she is and give her a treat. good luck!