Ooops!~I just went back to look at your previous post and you are the breeder. I'm glad that you had your little girl spayed after this litter since as I said previously AAI is a genetic issue. What happened to the rest of the puppies in the litter? Are you in contact with their owners? They need to be alerted to what is happening so that their puppies can be properly evaluated for AAI as well. I'm sorry