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Copied & Pasted from the link above. Written by Dr. Center: Additional Information; *It is no longer recommended to fast a dog for 12 hours before first collection or meal. The same size and type of meal that the dog normally eats is what should be used for the meal. *Test puppies at 16 weeks and again at 6 months. Dogs > 4 years of age can have other liver problems that will affect the Bile Acid test. *If the dog is stuffed or if the dog is frightened the test result can possibly be skewed. *The draw can be done one day pre feeding, then the dog can be taken home and fed and another draw done 2 hours post-feeding. It is not imperative it be done the same day.
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