This is an interesting thread
I have three females, the youngest, is the daughter of the middle female...and the smallest of the three. She is spayed, the largest oldest female is spayed, and the mother dog, is not spayed.
That being said--the youngest, smallest female is definitely the most dominant! (pictured in my avatar). She definitely lets the other two know it too..for instance, she will save her cookie treat, wait till the others come near, and give them "heck" for trying to even think about stealing it..taunting them even!
Glad my own three human children didn't play this game!!! (at least i didn't know about it

)
Debbie
Tasha, Tea' & Katie