Quote:
Originally Posted by Sunnie  no food.... water is always ok |
That is outdated protocol and no longer correct. You need paired samples around a meal with no fasting.
This is from Dr. Center:
"Dogs DO NOT need to be fasted for 12-hours to conduct this test. The important issue is to test BEFORE and 2-Hrs after FEEDING to fully evaluate the dogs ability to extract bile acids from the portal circulation."
PSVA and MVD Research Summary
"7. Random "Fasting" Bile Acids ARE NOT reliable for RULING OUT liver dysfunction or abnormal portal circulation. Rather, you need PAIRED SAMPLES AROUND A MEAL. We no longer collect 12-hr fasting bile acids but instead collect a bile acid sample before a meal (pre-meal) and 2 hours after a meal (post-meal or postprandial)." (all emphasis was added by Dr. Center, not me)