Yes, that can affect the pre-prandial (pre-meal) number, but the post-prandial (post-meal) number is the one that will indicate if the bile is clearing. If the bile is not clearing after meals, then it indicates a problem.
Yes, it is safe to bile acid test a 13 week old pup. 16 weeks is preferred, but 13 weeks is fine. In case your vet is not up to date on bile acid testing, it is no longer necessary to do a 12 hour fast as recommended previously. They just need to take paired samples. One before the meal and one two hours after the meal. This is recommended by Dr. Center from Cornell who is one of the leading experts in liver shunt and actually invented the bile acid test.
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