i don't do it on my puppies. But my adult dogs have all had it done when a few months old. My vet refuses to do it because of the fasting. he wouldn't do it on Ceeby at ten weeks old and now won't do it on my puppies. he is well aware that there are tests that can be done without fasting them but these tests usually require a repeat test at a few months old so it is just better in his opinion to wait until they are old enough and weigh enough that fasting isn't an issue and get a more accurate test. According to him, not me.