Right now I am treating some older stains for the 2nd time-I put it down 2 days ago-it is now hard and again there is yellow coloring so it obviously picked up more urine. I will see if 2 treatments are enough after this. It truly is amazing to see the yellow mound and u can smell the urine in it-I;m keeping my fingers crossed this will at least clean up the major spots. We just had our closet redone this weekend and when we emptied it out for the contractors we found little pp spots where our shoes were-I had to laugh so that is the next room and they look old. She is such a stinker